Incontinence during day or night time or urinary tract problems resulting in enuresis which is assumed to be occurred by either of three known pathophysiology which includes detrusor over activity, nocturnal polyuria and arousal thresholds is in modern terminology called as Nocturnal Enuresis (NE). Drugs like desmopressin, can be used as corrective treatment for polyuria which is evidently known as antienuretic drug, but it is not universally clear on therapeutic efficacy. Based on therapeutic efficacy logics, the anticholinergic drugs are estimated to be effective for treating detrusor dependent NE, but it is yet not clear, due to lack of firm evidence. Study was carried out in a tertiary care hospital under the supervision of pediatrician after getting approval from the institutional ethics committee of the study hospital. All the materials required for the study have been collected from the pediatric department. On examination followed by various laboratory techniques, it was evaluated as shown in the Table 2, which included nocturnal enuresis as 37.12 mg/g creatinine, nocturnal enuresis plus diurnal incontinence as 71.45 mg/g creatinine, and in control group it was 24.56 mg/g creatinine. It is clearly seen that there is no significant relation with familial history in children acquired with NE or DI. The study shown a slight relation between NE and ADHD. However, further deep research is required with huge study population to evaluate and estimate the evidences.